Immune Checkpoint Antibodies
Immune checkpoints are regulators of the immune system which prevent the immune system from attacking self-antigens indiscriminately. While this is critical to preventing auto-immune disease it also causes the immune system to be ineffective in eradicating or suppressing cancer. Tumor cells exploit certain immune-checkpoint pathways as a major mechanism of immune resistance, particularly against T cells that are specific for tumor antigens. Because many of these immune checkpoints are initiated by ligand-receptor interactions, they can be readily blocked by antibodies. Blocking these immune checkpoints allows antitumor activity to resume and is among the most promising approaches to activating therapeutic anti-tumor immunity. Bio X Cell offers an extensive selection of antibodies targeting mouse immune checkpoint proteins. Click the link below to shop all Immune Checkpoint blocking antibodies or finding your target of interest by exploring the interactive map below.Ā
4-1BB is a 39 kDa transmembrane protein expressed by T lymphocytes, NK cells, dendritic cells, granulocytes, and mast cells. Upon binding its ligand 4-1BBL, 4-1BB provides costimulatory signals to both CD4 and CD8 T cells through the activation of NF- ĪŗB, c-Jun and p38 downstream pathways. The importance of the 4-1BB pathway has been underscored in a number of diseases, including cancer. Agonistic anti-4-1BB antibodies have been reported to induce T cell-mediated antitumor immunity.
AVAILABLE ANTI-MOUSE 4-1BB ANTIBODIES:
Clone LOB12.3 - This clone was first published in 2002 by Vadim Y. Taraban et al. The immunogen used to create this antibody is reported as a mouse CD137 human Fc fusion protein. The original publication describing the generation of this antibody can be found here.
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InVivoMAb anti-mouse 4-1BB (CD137)
InVivoPlus anti-mouse 4-1BB (CD137)
Clone 3H3 - This clone was first published in 1997 by Walter W. Shuford et al. The immunogen used to create this antibody is reported as a mouse CD137 human Fc fusion protein. The original publication describing the generation of this antibody can be found here.
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InVivoMAb anti-mouse 4-1BB (CD137)
Clone 17B5 - Origin information unknown
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4-1BBL is a 97 kDa member of the TNF superfamily and is expressed by dendritic cells, macrophages, and activated B and T lymphocytes. Interaction of 4-1BBL with 4-1BB (CD137) provides costimulatory signals to both CD4 and CD8 T cells through the activation of NF-ĪŗB, c-Jun, and p38 downstream pathways.
AVAILABLE ANTI-MOUSE 4-1BBL ANTIBODIES:
Clone TKS-1 - This clone was first published in 2002 by Toshiro Futagawa et al. The immunogen used to create this antibody is reported as mouse 4-1BBL transfected NRK cells. The original publication describing the generation of this antibody can be found here.
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BTLA is an Ig superfamily member which is expressed on B cells, T cells, macrophages, dendritic cells, NK cells, and NKT cells. Like PD-1 and CTLA-4, BTLA interacts with a B7 homolog, B7-H4. However, unlike PD-1 and CTLA-4, BTLA displays T cell inhibition via interaction with tumor necrosis family receptors, not just the B7 family of cell surface receptors. BTLA is a ligand for herpes virus entry mediator (HVEM). BTLA-HVEM complexes have been shown to negatively regulate T cell immune responses.
AVAILABLE ANTI-MOUSE BTLA ANTIBODIES:
Clone 6A6 - Origin information unknown
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InVivoMAb anti-mouse BTLA (CD272)
Clone PK18.6 - This clone was first published in 2004 by Peggy Han et al. The immunogen used to create this antibody is reported as mouse BTLA external domain-human IgG fusion protein. The original publication describing the generation of this antibody can be found here.
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InVivoMAb anti-mouse BTLA (CD272)
Clone PJ196 - This clone was first published in 2005 by Carsten Krieg et al. The immunogen used to create this antibody is unknown. The original publication describing the generation of this antibody can be found here.
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CD27 is a 45 kDa type I transmembrane protein and a member of the TNF superfamily. CD27 is expressed on peripheral T cells, memory B cells, NK cells, and a subset of thymocytes. CD27 is highly induced on T cells after TCR stimulation. CD70 is a ligand for CD27. Interaction with CD70 provides co-stimulation in T cells and induces signaling events essential for cell proliferation, long-term maintenance of antigen-specific T cells, antiviral responses, antitumor immunity, and alloreactivity. Agonistic antibodies that stimulate CD27 are currently being explored as experimental cancer treatments.
AVAILABLE ANTI-MOUSE CD27 ANTIBODIES
Clone RM27-3E5 - This clone was created in 2010 by Dr. Hideo Yagita. The immunogen used to create this antibody is reported as a mouse CD27-human IgG1 Fc fusion protein. The original publication describing the generation of this antibody can be found here.
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CD28 is expressed by thymocytes, most peripheral T cells, and NK cells. CD28 is a receptor for CD80 (B7-1) and CD86 (B7-2). Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.
AVAILABLE ANTI-MOUSE CD28 ANTIBODIES:
Clone 37.51 - This clone was first published in 1992 by J A Gross et al. The immunogen used to create this antibody is reported as C57BL/6 mouse T cell lymphoma EL-4 cells. The original publication describing the generation of this antibody can be found here.
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Clone PV-1 - This clone was first published in 1995 by R Abe et al. The immunogen used to create this antibody is reported as C57BL/6 mouse T cell lymphoma EL-4 cells. The original publication describing the generation of this antibody can be found here.
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Clone D665 - This clone was first published in 2006 by Kevin M. Dennehy et al. The immunogen used to create this antibody is reported as A20 cells expressing mouse CD28 and a recombinant mouse CD28-Ig fusion protein. The original publication describing the generation of this antibody can be found here.
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CD40 is expressed broadly on antigen-presenting cells (APCs) such as dendritic cells, B cells, macrophages, and monocytes as well as non-immune endothelial cells, basal epithelial cells, and a range of tumors. Upon binding to its ligand CD154, CD40 acts as a costimulatory molecule for the activation of B cells, dendritic cells, monocytes, and other APCs. CD40 plays roles in B cell activation, differentiation, proliferation and Ig isotype switching as well as dendritic cell maturation. Agonistic CD40 monoclonal antibodies have been shown to activate APCs and promote anti-tumor T cell responses.
AVAILABLE ANTI-MOUSE CD40 ANTIBODIES:
Clone FGK4.5 - This clone was created in 1996 by Drs. J. Andersson and A. Rolink. The immunogen used to create this antibody is reported as recombinant mouse CD40 fusion protein. The original publication describing the generation of this antibody can be found here.
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CD154 is primarily expressed on the surface of activated CD4+ T lymphocytes but can also be expressed by platelets, mast cells, macrophages, basophils, NK cells, B lymphocytes, CD8+ T lymphocytes as well as non-hematopoietic cells including smooth muscle cells, endothelial cells, and epithelial cells. CD154 signals through CD40 and is thought to play a key role in T and B lymphocyte costimulation. Anti-CD154 antibodies have been reported to inhibit the formation of germinal centers and disrupt antigen-specific T cell responses.
AVAILABLE ANTI-MOUSE CD40L ANTIBODIES:
Clone MR-1 - This clone was first published in 1992 by Dr. Randolph. J. Noelle et al. The immunogen used to create this antibody is reported as activated mouse TH1 clone D1.6. The original publication describing the generation of this antibody can be found here.
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Mouse and human CD44 also known as Hermes, HCAM, and Pgp-1. CD44 is an 80-95 kDa glycoprotein that is expressed on all leukocytes, endothelial cells, hepatocytes, and mesenchymal cells. As an adhesion molecule, CD44 participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing, and hematopoiesis. CD44 is a receptor for hyaluronic acid and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Additionally, CD44 is involved in tumor metastasis and targeting of CD44 by antibodies has been shown to reduce the malignant activities of various neoplasms. Interestingly, high levels of the adhesion molecule CD44 on leukemic cells are essential to generate leukemia.Ā
AVAILABLE ANTI-MOUSE CD44 ANTIBODIES:
Clone IM7 - This clone was first published in 1982 by Ian S. Trowbridge et al. The immunogen used to create this antibody is reported as dexamethasone-induced myeloid leukemia M1 cells. The original publication describing the generation of this antibody can be found here.
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InVivoMAb anti-mouse/human CD44
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CD47 is ubiquitously expressed by both hematopoietic cells such as T and B lymphocytes, monocytes, platelets and erythrocytes and non-hematopoietic cells. CD47 is involved in a range of cellular processes, including apoptosis, proliferation, adhesion, and migration. Furthermore, it plays a key role in immune and angiogenic responses. CD47 is a receptor for thrombospondin-1 (TSP-1), a secreted glycoprotein that plays a role in vascular development and angiogenesis. CD47 Is has been found to be overexpressed in many different tumor cells. Because of this, anti-CD47 monoclonal antibodies have been proposed and studied as a therapeutic treatment for human cancers.
AVAILABLE ANTI-MOUSE CD47 ANTIBODIES:
Clone MIAP301 - Origin information unknown
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InVivoMAb anti-mouse CD47 (IAP)
Clone MIAP410 - This clone was first published in 2000 by Xin Han et al. The immunogen used to create this antibody is reported as purified human placental CD47. The original publication describing the generation of this antibody can be found here.
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CD70 is expressed by activated mouse T and B lymphocytes and dendritic cells. CD70 is a ligand for CD27 and their interaction promotes T and B cell cross-stimulation and co-stimulation of B cell proliferation and immunoglobulin production. Cells expressing CD70 have been shown to co-stimulate T cell proliferation and induce cytokine production.
AVAILABLE ANTI-MOUSE CD70 ANTIBODIES:
Clone FR70 - This clone was first published in 1998 by H Oshima et al. The immunogen used to create this antibody is reported as BALB/c mouse B lymphoma A20.2J. The original publication describing the generation of this antibody can be found here.
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CD80 is expressed by activated B cells and constitutively by monocytes and dendritic cells. This ligand binds to CD28 to provide a costimulatory signal necessary for T cell activation and survival, and cytokine production. Additionally, CD80 binds to CTLA-4 which inhibits T cells.
AVAILABLE ANTI-MOUSE CD80 ANTIBODIES:
Clone 1G10 - This clone was first published in 1992 by Ziba Razi-Wolf et al. The immunogen used to create this antibody is reported as dibutyryl cAMP-Activated 5C2 cells. The original publication describing the generation of this antibody can be found here.
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InVivoMAb anti-mouse CD80 (B7-1)
Clone 16-10A1 - This clone was first published in 1994 by Gordon D.Powers et al. The immunogen used to create this antibody is reported as CHO cells transfected with mouse CD80. The original publication describing the generation of this antibody can be found here.
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CD86 is expressed by activated T and B cells, macrophages, and dendritic cells. This ligand binds to CD28 to provide a costimulatory signal necessary for T cell activation and survival, and cytokine production. Additionally, CD80 binds to CTLA-4 which inhibits T cells.
AVAILABLE ANTI-MOUSE CD86 ANTIBODIES:
Clone GL-1 - This clone was first published in 1993 by Karen S. Hathcock et al. The immunogen used to create this antibody is reported as LPS-activated CBA/Ca mouse splenic B cells. The original publication describing the generation of this antibody can be found here.
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CD200 is a type 1 membrane glycoprotein belonging to the immunoglobulin superfamily. CD200 expression is limited to thymocytes, neurons, B cells, splenic follicular dendritic cells and endothelium, and subsets of T cells and dendritic cells. CD200 has been shown to co-stimulate T cell proliferation. It is thought that engagement of CD200 with its receptor, CD200R, results in inhibition and/or downregulation of myeloid cell activity. Blocking this interaction decreases the inhibitory thresholds of myeloid cells resulting in increased immune activity.
AVAILABLE ANTI-MOUSE CD200 ANTIBODIES:
Clone OX-90Ā - This clone was first published in 2000 by Robert M. Hoek et al. The immunogen used to create this antibody is reported as a fusion protein consisting of mouse CD200 (extracellular region) and rat CD4 (domains 3 and 4). The original publication describing the generation of this antibody can be foundĀ here.
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CD276 is expressed weakly on activated lymphocytes, macrophages, dendritic cells, nasal and airway epithelial cells, osteoblasts, and some tumor cell lines. A soluble form of CD276 is also secreted by monocytes, dendritic cells, and activated T cells. The biological role of CD276 is still under investigation however, recent studies suggest a negative regulatory role for CD276 in T cell responses.
AVAILABLE ANTI-MOUSE CD276 ANTIBODIES:
Clone MJ18 - This clone was first published in 2008 by Osamu Nagashima et al. The immunogen used to create this antibody is reported as a mouse B7-H3 IgG2a fusion protein. The original publication describing the generation of this antibody can be found here.
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CTLA-4 is expressed on activated T and B lymphocytes. CTLA-4 is structurally similar to the T-cell co-stimulatory protein, CD28, and both molecules bind to the B7 family members B7-1 (CD80) and B7-2 (CD86). Upon ligand binding, CTLA-4 negatively regulates cell-mediated immune responses. CTLA-4 plays roles in induction and/or maintenance of immunological tolerance, thymocyte development, and regulation of protective immunity. The critical role of CTLA-4 in immune down-regulation has been demonstrated in CTLA-4 deficient mice, which succumb at 3-5 weeks of age due to the development of a lymphoproliferative disease. CTLA-4 is among a group of inhibitory receptors being explored as cancer treatment targets through immune checkpoint blockade.
AVAILABLE ANTI-MOUSE CTLA-4 ANTIBODIES:
Clone 9H10 - This clone was first published in 1995 by Matthew F. Krummel and James P. Allison. The immunogen used to create this antibody is reported as mouse CTLA-4-human IgG1 fusion protein. The original publication describing the generation of this antibody can be found here.
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InVivoMAb anti-mouse CTLA-4 (CD152)
InVivoPlus anti-mouse CTLA-4 (CD152)
Clone 9D9 - Origin information unknown
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InVivoMAb anti-mouse CTLA-4 (CD152)
InVivoPlus anti-mouse CTLA-4 (CD152)
Clone UC10-4F10-11 - This clone was first published in 1994 by Theresa L. Walunas. The immunogen used to create this antibody is reported as mouse CTLA-4 IgG2a fusion protein. The original publication describing the generation of this antibody can be found here.
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FGL-1 is a member of the fibrinogen family of proteins. Under normal physiological conditions, FGL-1 is primarily secreted from hepatocytes and contributes to mitogenic and metabolic functions. FGL-1 is produced at high levels in various tumors including lung cancer and melanoma. High FGL-1 expression is associated with resistance to anti-PD-1/PD-L1 therapy and poor prognosis of cancer patients. Recently, FGL-1 has been identified as a major inhibitory ligand for LAG-3, a receptor that negatively regulates the proliferation, activation, and effector function of T cells.
AVAILABLE ANTI-MOUSE FGL-1 ANTIBODIES:
Clone 177R4 - This clone was first published in 2018 by Jun Wang et al. The immunogen used to create this antibody is reported as murine FGL-1-Ig fusion protein. The original publication describing the generation of this antibody can be found here.
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GITR is expressed at low levels on resting T lymphocytes and at high levels on regulatory T cells. GITR is upregulated on activated T cells where it provides co-stimulation. GITR ligand (GITRL) is found on B cells, macrophages, dendritic and endothelial cells, and is implicated in regulating both innate and adaptive immune responses. GITR is also thought to play a key role in dominant immunological self-tolerance maintained by regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T cell activation and programmed cell death.
AVAILABLE ANTI-MOUSE GITR ANTIBODIES:
Clone DTA-1 - This clone was first published in 2002 by Jun Shimizu et al. The immunogen used to create this antibody is reported as mouse CD25+ CD4+ T cells. The original publication describing the generation of this antibody can be found here.
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Galectin-9 binds to Ī²-galactosides and can serve as a ligand for TIM-3 (CD366). The protein is implicated in both innate and adaptive immune responses specifically, induction of cytokine secretion by macrophages, bactericidal functions, promotion of dendritic cell maturation, regulatory T cell expansion, and negative regulation of Th1, Th17, NK, and cytotoxic T cells.
AVAILABLE ANTI-MOUSE GALECTIN-9 ANTIBODIES:
Clone RG9-1 - This clone was first published in 2008 by Atsuki Fukushima et al. The immunogen used to create this antibody is reported as recombinant mouse galectin-9. The original publication describing the generation of this antibody can be found here.
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ICOSL (inducible T cell co-stimulator ligand) also known as CD275, B7RP-1, and B7-H2. ICOSL is a 40 kDa immune checkpoint protein belonging to the Ig receptor superfamily. Upon ICOSL binding, ICOS signaling co-stimulates T and B cell responses. The ligand Is expressed on antigen presenting cells including splenic B cells, dendritic cells, and macrophages. ICOS signaling is also thought to be important for maintaining regulatory T cell homeostasis.
AVAILABLE ANTI-MOUSE ICOSL ANTIBODIES:
Clone HK5.3 - This clone was first published in 2002 by Hideyuki Iwai et al. The immunogen used to create this antibody is reported as mouse B7-RP1 transfected cell line. The original publication describing the generation of this antibody can be found here.
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ICOS is a 47-57 kDa homodimeric glycoprotein belonging to the CD28 family of costimulatory molecules. ICOS is expressed on activated T cells and upon ICOSL binding, co-stimulates T and B cell responses. The ligand Is expressed on antigen presenting cells including splenic B cells, dendritic cells, and macrophages. ICOS signaling is also thought to be important for maintaining regulatory T cell homeostasis.
AVAILABLE ANTI-MOUSE ICOS ANTIBODIES:
Clone 7E.17G9 - This clone was first published in 2000 by Alexander J. McAdam et al. The immunogen used to create this antibody is reported as mouse ICOS cDNA and ICOS hexahistidine fusion protein. The original publication describing the generation of this antibody can be found here.
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LAG-3 is expressed by activated T lymphocytes, NK cells, and T regulatory cells. LAG-3's main ligand is MHC class II which it binds to with a higher affinity than even CD4 does. Upon binding LAG-3 is thought to play similar roles as CTLA-4 and PD-1 including downregulation of TCR signaling and inhibition of CD4-dependent T cell function. LAG-3 has also been demonstrated to contribute to the suppressor function of T regulatory cells. In contrast to inhibition, LAG-3 has been shown to promotes immune responses by activating antigen-presenting cells.
AVAILABLE ANTI-MOUSE LAG-3 ANTIBODIES:
Clone C9B7W - This clone was first published in 2002 by Creg J. Workman et al. The immunogen used to create this antibody is reported as a mouse CD223-Ig fusion protein. The original publication describing the generation of this antibody can be found here.
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Osteopontin is a secreted arginine-glycine-aspartic acid (RGD)-containing glycoprotein that was originally isolated from bone. Osteopontin has been found in kidney, vascular tissues, biological fluids, and various tumor tissues. Osteopontin interacts with integrins and CD44 and regulates diverse biological processes including bone development, immune responses, and oncogenesis. Osteopontin is elevated in human colorectal cancer and is thought to function as an immune checkpoint.
AVAILABLE ANTI-MOUSE Osteopontin ANTIBODIES:
Clone 103D6Ā - This clone was first published in 2021 by John D. Klement et al. The immunogen used to create this antibody is reported as recombinant mouse OPN protein. The original publication describing the generation of this antibody can be foundĀ here.
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InVivoMAb anti-mouse osteopontin (SPP1)
Clone 100D3Ā - This clone was first published in 2021 by John D. Klement et al. The immunogen used to create this antibody is reported as recombinant mouse OPN protein. The original publication describing the generation of this antibody can be foundĀ here.
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OX-40 is expressed on activated CD4 and CD8 T cells but is not found on resting naĆÆve T cells or most resting memory T cells. Although it was originally thought that OX-40 expression was restricted to activated conventional T cells, it has now been visualized on activated regulatory T cells, NKT cells, NK cells, and neutrophils. OX-40 plays a major role in regulating both CD4 and CD8 T cell clonal expansion. It provides a costimulatory signal to antigen-reacting naive T cells to prolong proliferation, as well as augment the production of several cytokines. This is demonstrated by OX-40 knockout mice which generate fewer primary effector CD4 T cells after immunization. Furthermore, in vivo treatment with an agonist antibody to OX-40 has been shown to strongly enhance the generation of antigen-specific effector T cells and prevent the induction of T cell tolerance.
AVAILABLE ANTI-MOUSE OX40 ANTIBODIES:
Clone OX-86 - This clone was first published in 1996 by Aymen Al-Shamkhani et al. The immunogen used to create this antibody is reported as recombinant mouse OX40-CD4 chimeric protein. The original publication describing the generation of this antibody can be found here.
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OX40L is expressed on activated B cells and antigen presenting cells. OX40L is the ligand for OX40 (CD134). OX40 signaling regulates both CD4 and CD8 T cell clonal expansion. It provides a costimulatory signal to antigen-reacting naive T cells to prolong proliferation, as well as augment the production of several cytokines including IL-2.
AVAILABLE ANTI-MOUSE OX40L ANTIBODIES:
Clone RM134L - This clone was first published in 1999 by Yoshinori Seko et al. The immunogen used to create this antibody is reported as rat NRK-52E cells transfected with mouse OX40L. The original publication describing the generation of this antibody can be found here.
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PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. Additionally, Pdcd1 mRNA is expressed in developing B lymphocytes during the pro-B-cell stage. PD-1's structure includes an ITIM (immunoreceptor tyrosine-based inhibitory motif) suggesting that PD-1 negatively regulates TCR signals. PD-1 signals via binding its two ligands, PD-L1 and PD-L2 both members of the B7 family. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Additionally, PD-1 is known to play key roles in peripheral tolerance and prevention of autoimmune disease in mice as PD-1 knockout animals show dilated cardiomyopathy, splenomegaly, and loss of peripheral tolerance. Induced PD-L1 expression is common in many tumors including squamous cell carcinoma, colon adenocarcinoma, and breast adenocarcinoma. PD-L1 overexpression results in increased resistance of tumor cells to CD8 T cell-mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and its receptor PD-1. For these reasons anti-PD-1 mediated immunotherapies are currently being explored as cancer treatments.
AVAILABLE ANTI-MOUSE PD-1 ANTIBODIES:
Clone RMP1-14 - This clone was first published in 2003 by Takanori Kanai et al. The immunogen used to create this antibody is reported as Syrian hamster BKH cells transfected with mouse PD-1 cDNA. The original publication describing the generation of this antibody can be found here.
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InVivoMAb anti-mouse PD-1 (CD279)
InVivoPlus anti-mouse PD-1 (CD279)
Clone 29F.1A12 - This clone was first published in 2003 by Spencer C. Liang et al. The immunogen used to create this antibody is reported as a recombinant mouse PD-1-Ig fusion protein. The original publication describing the generation of this antibody can be found here.
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InVivoMAb anti-mouse PD-1 (CD279)
InVivoPlus anti-mouse PD-1 (CD279)
Clone J43 - This clone was first published in 1996 by Yasutoshi Agata et al. The immunogen used to create this antibody is reported as Syrian hamster BKH cells transfected with mouse PD-1 cDNA. The original publication describing the generation of this antibody can be found here.
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PD-L1 is expressed on T lymphocytes, B lymphocytes, NK cells, dendritic cells, as well as IFNĪ³ stimulated monocytes, epithelial cells, and endothelial cells. PD-L1 binds to its receptor, PD-1, found on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Engagement of PD-L1 with PD-1 leads to inhibition of TCR-mediated T cell proliferation and cytokine production. PD-L1 is thought to play an important role in tumor immune evasion. Induced PD-L1 expression is common in many tumors and results in increased resistance of tumor cells to CD8 T cell-mediated lysis. In mouse models of melanoma, tumor growth can be transiently arrested via treatment with antibodies which block the interaction between PD-L1 and PD-1.
AVAILABLE ANTI-MOUSE PD-L1 ANTIBODIES:
Clone 10F.9G2 - This clone was first published in 2002 by Michael J. Eppihimer et al. The immunogen used to create this antibody is reported as CHO-mPD-L1 transfectants. The original publication describing the generation of this antibody can be found here.
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PD-L2 is a 25 kDa type I transmembrane protein that belongs to the B7 family of the Ig superfamily. PD-L2 is expressed on monocytes, macrophages, and subsets of dendritic cells. PD-L2 binds to its receptor, PD-1, found on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. Engagement of PD-L2 with PD-1 leads to inhibition of TCR-mediated T cell proliferation and cytokine production.
AVAILABLE ANTI-MOUSE PD-L2 ANTIBODIES:
Clone TY25 - This clone was first published in 2002 by Tomohide Yamazaki et al. The immunogen used to create this antibody is reported as mouse B7-DC-transfected RAW264.7 cells. The original publication describing the generation of this antibody can be found here.
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PSGL-1 is a 230 kDa glycoprotein that is expressed by bone marrow-derived mast and dendritic cells, splenic leukocytes, platelets, peripheral blood neutrophils, and T lymphocytes. PSGL-1 is a ligand for P-selectin (CD62P) and plays roles in leukocyte rolling, the migration of leukocytes into inflamed tissues, and responses to vascular injury.
AVAILABLE ANTI-MOUSE PSGL-1 (CD162) ANTIBODIES:
Clone 4RA10 - This clone was first published in 2017 by Gunther G. Pendl et al. The immunogen used to create this antibody is reported as mouse PSGL-1 human IgG1 fusion protein. The original publication describing the generation of this antibody can be found here.
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RGMs are GPI-anchored membrane proteins that bind bone morphogenetic proteins (BMPs) and neogenin. BMPs have diverse roles in many processes, including cell proliferation, differentiation, and apoptosis. RGMb has a well-elucidated role in neural development, with RGMb knock-out mice dying 2-3 weeks after birth. RGMb is also expressed by macrophages and can regulates IL-6 expression. Recently, it has been shown that PD-L2 also binds to RGMb and that blocking the RGMbāPD-L2 interaction impairs the development of respiratory tolerance but blocking the PD-1āPD-L2 interaction does not.
AVAILABLE ANTI-MOUSE RGMb ANTIBODIES:
Clone 307.9D1 - This clone was first published in 2014 by Yanping Xiao et al. The immunogen used to create this antibody is reported as recombinant murine RGMb. The original publication describing the generation of this antibody can be foundĀ here.
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TIGIT is a 26 kDa, type I transmembrane protein and a member of the poliovirus receptor (PVR) family. TIGIT has been found to be expressed on follicular T helper cells in mice while in humans it's expressed by many T cell subsets including activated T cells, follicular T helper cells, memory T cells, and regulatory T cells as well as on NK cells. TIGIT can interact with certain members of the PVR and PVR-like families, including PVR, PVRL2, PVRL3, CD155, and CD112. TIGIT is thought to negatively regulate NK and T cell activation. Binding of TIGIT on T cells by dendritic cells results in their differentiation into a tolerogenic phenotype, with increased secretion of IL-10 and diminished production of IL-12. TIGIT knock-out mice are more susceptible to autoimmune disease.
AVAILABLE ANTI-MOUSE TGIT ANTIBODIES:
Clone 1G9 - This clone was first published in 2011 by Nicole Joller et al. The immunogen used to create this antibody is reported as mouse mouse TIGIT protein. The original publication describing the generation of this antibody can be found here.
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TIM-1 is a type I cell-surface glycoprotein and member of the Ig superfamily. TIM-1 is preferentially expressed on TH2 cells and has been identified as a stimulatory molecule for T cell activation. The TIM gene family plays critical roles in regulating the immune response to viral infection. TIM-1 is also involved in allergic responses, asthma, and transplant tolerance.
AVAILABLE ANTI-MOUSE TIM-1 ANTIBODIES:
Clone RMT1-10 - This clone was first published in 2006 by Atsuki Fukushima et al. The immunogen used to create this antibody is reported as a full-length mouse TIM-1-Ig fusion protein. The original publication describing the generation of this antibody can be found here.
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InVivoMAb anti-mouse TIM-1 (CD365)
Clone 3B3 - This clone was first published in 2005 by Sarah E Umetsu et al. The immunogen used to create this antibody is reported as a mouse TIM-1 (signal and IgV domains)/mouse IgG2a Fc fusion protein. The original publication describing the generation of this antibody can be found here.
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InVivoMAb anti-mouse TIM-1 (CD365)
Clone 3D10 - This clone was first published in 2005 by Sarah E Umetsu et al. The immunogen used to create this antibody is reported as a mouse TIM-1 (signal and IgV domains)/mouse IgG2a Fc fusion protein. The original publication describing the generation of this antibody can be found here.
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TIM-3 is specifically expressed at high levels on the surface of Th1 lymphocytes whereas Th2 lymphocytes express TIM-1 and TIM-2. TIM-3 activation occurs via binding to the cell-associated C-type lectin galectin-9. Upon binding TIM-3 induces apoptosis of Th1 cells. Inhibition of TIM-3 signaling in mice has been shown to exacerbate experimental autoimmune encephalomyelitis, promote IFNĪ³ production and Th1 cell proliferation. Tim-3 has also been shown to be required for the induction of tolerance, as both TIM-3 knockout animals and mice treated with TIM-3-Ig fusion protein display defects in the induction of antigen-specific tolerance. Additionally, TIM-3 signaling is currently being explored as a cancer immunotherapy target as CD8 T cells which express both TIM-3 and PD-1 exhibit greater defects in both cell-cycle progression and effector cytokine production than cells that express PD-1 alone.
AVAILABLE ANTI-MOUSE TIM-3 ANTIBODIES:
Clone RMT3-23 - This clone was first published in 2006 by Tsunekazu Oikawa et al. The immunogen used to create this antibody is reported as recombinant mouse TIM-3. The original publication describing the generation of this antibody can be found here.
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InVivoMAb anti-mouse TIM-3 (CD366)
InVivoPlus anti-mouse TIM-3 (CD366)
Clone B8.2C12 - Origin information unknown
Additional product information and references:
V-domain Ig suppressor of T cell activation (VISTA), also known as PD-1H and B7-H5, is a 309 aa type I transmembrane glycoprotein and a member of the Ig superfamily. VISTA is expressed on naĆÆve and activated T cells, NK cells, macrophages, dendritic cells, and neutrophils. VISTA functions as a negative immune-checkpoint protein that suppresses T cell cytokine production and proliferation. VISTA is overexpressed by tumor-infiltrating lymphocytes, such as myeloid cells and regulatory T cells. Blockade of VISTA results in delayed tumor growth in mouse models of melanoma.
AVAILABLE ANTI-MOUSE VISTA ANTIBODIES:
Clone 13F3 - This clone was first published in 2011 by Li Wang et al. The immunogen used to create this antibody is reported as EL4 cells overexpressing mouse VISTA-RFP followed by a boost with VISTA-Ig fusion protein. The original publication describing the generation of this antibody can be found here.
Additional product information and references: